Using our human pancreatic islet cohort of from lean, obese and T2D subjects we offer:
- Target and Untarget gene expression: qPCR, Digital PCR
- Protein expression: Western Blot, Immunoflourescence and Immunochemical studies
- Cell composition and arrangement, morphology analysis: immunoistochemistry, immunofluorescence and confocal imaging
Using human islets isolated by our team of experts we perform:
- In vitro tailored mechanist and intracellular signaling studies
- Human islet function assessment by glucose-stimulated dynamic hormone secretion: perifusion
- In vitro drug function and toxicity testing
- siRNA gene silencing to study a specific gene loss-of-function
Using the human islet transplantation in immunodeficient mice (QIVIPA) we offer:
- Evaluation of human graft function in vivo
- Assessement of human graft vascularisation and innervation: 3D iDisco technique
- Assessement of human graft response or adaptation to drug treatment in vivo
- Metabolic profiling of the oral glucose challenge or standardized meal test in vivo
